anti mouse cd3  (Bio-Rad)

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: CD8 + T cells promote tubule-interstitial damage in malaria-induced acute kidney injury

doi: 10.3389/fcimb.2025.1561806

Figure Lengend Snippet: Malaria-responsive T cells migrate to target organs. (A) Schematic representation of experimental design. Donor animals were infected 5 days before adoptive transfer. T cells from the donors’ spleens were enriched in wool columns and transferred to naive acceptor animals, generating two groups: acceptors that received T cells from uninfected donors (Naive → Naive); acceptors that received T cells from infected donors (Infected → Naive). On the third day after adoptive transfer, T cell migration was analyzed, and renal function was assessed. (B) Parasitemia was observed only in donor infected mice (gray bar) (n = 6–8). (C) Migration of malaria-responsive T cells to the kidney by CD3 + /CFSE + cells (n = 4). (D) To confirm migration to the kidney, C57BL/6-GFP animals, infected or not, were used as donors to perform adoptive transfer (n = 6). Migration is expressed as the percentage of CD3 + /CFSE + or CD3 + /GFP + cells relative to the total CD3 + cells in the organ of interest. *versus naive → naive group, P < 0.05.

Article Snippet: For T cell migration experiments, the cell suspension was incubated with a PeCy5.5-conjugated hamster IgG1 anti-murine CD3 antibody (145-2C11, eBioscience, San Diego, CA, USA).

Techniques: Infection, Adoptive Transfer Assay, Migration